Barley elongation factor 1 alpha: genomic organization, DNA sequence, and phylogenetic implications

Abstract

A full length cDNA clone encoding the 447 amino acid long barley (Hordeum vulgare cv. Bomi) endosperm elongation factor 1 alpha (eF-1 alpha) was isolated by a differential screening procedure. RFLP mapping of eF-1 alpha showed that the barley genome contains a small eF-1 alpha gene family of 4 copies, with 1 copy of the gene being located on each of chromosomes 2, 4, 6, and 7. Analysis of barley endosperm total proteins by Western blot with antibodies directed towards wheat eF-1 alpha and the sea urchin 51 kDa proteins gave a single band of the expected molecular weight. Amino acid sequence comparison with other plant eF-1 alpha sequences showed that the isolated barley endosperm eF-1 alpha is more similar to the published wheat eF-1 alpha sequence than to eF-1 alpha sequences previously published for the barley cultivars Igri and Dicktoo. The phylogenetic analysis suggests that the barley eF-1 alpha gene family can be divided into two subfamilies and that two ancestral genes existed before the divergence of monocotyledonous and dicotyledonous plants.