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. 1997 Jul;172(1-2):67-79.

Identification of proteins that interact with a protein of interest: applications of the yeast two-hybrid system

R D Gietz  1 , B Triggs-Raine, A Robbins, K C Graham, R A Woods
Affiliations
  • PMID: 9278233

Identification of proteins that interact with a protein of interest: applications of the yeast two-hybrid system

R D Gietz et al. Mol Cell Biochem. 1997 Jul.

Abstract

The yeast two-hybrid system is a molecular genetic test for protein interaction. Here we describe a step by step procedure to screen for proteins that interact with a protein of interest using the two-hybrid system. This process includes, construction and testing of the bait plasmid, screening a plasmid library for interacting fusion proteins, elimination of false positives and deletion analysis of true positives. This procedure is designed to allow investigators to identify proteins and their encoding cDNAs that have a biologically significant interaction with your protein of interest.

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