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. 1997 Jul;172(1-2):89-95.

Competitive RT-PCR for studying gene expression in micro biopsies

R Hullin  1 , F Asmus, G Steinbeck
Affiliations
  • PMID: 9278235

Competitive RT-PCR for studying gene expression in micro biopsies

R Hullin et al. Mol Cell Biochem. 1997 Jul.

Abstract

The technique of RT-PCR has become a powerful method for studying gene expression in various species and tissues. Here we present a competitive PCR for quantitative measurement of absolute levels of mRNA molecules for the regulatory alpha 2/delta subunit of the high-voltage-activated dihydropyridine sensitive human cardiac L-type calcium channel. With the method described the dynamic process of cardiac gene expression can be studied in human endomyocardial biopsies in different patient collectives and even in the individual.

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