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. 1977 Oct;4(10):3341-55.
doi: 10.1093/nar/4.10.3341.

A simple method for the preparation of [beta-32P]purine nucleoside triphosphase

Y Furuichi, A J Shatkin
Free PMC article

A simple method for the preparation of [beta-32P]purine nucleoside triphosphase

Y Furuichi et al. Nucleic Acids Res. 1977 Oct.
Free PMC article

Abstract

A rapid, simple and inexpensive procedure is described for the preparation of purine ribo-and deoxyribonucleoside triphosphates specifically and highly radiolabeled with [32P]phosphate in the beta position. The method involves two successive enzymatic reactions: conversion of donor [gamma-32P]ATP in the presence of an excess of acceptor 5'-mononucleotide to the 5'-diphosphates by myokinase or guanosine 5'-monophosphate kinase followed by phosphorylation with pyruvate kinase to yield 5'-triphosphates.

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References

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