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. 1977 Oct;4(10):3581-7.
doi: 10.1093/nar/4.10.3581.

Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells

D Skup, S Millward
Free PMC article

Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells

D Skup et al. Nucleic Acids Res. 1977 Oct.
Free PMC article

Abstract

Micrococcal nuclease was used to eliminate endogenous protein synthesis in extracts prepared from L cells. The nuclease can be inhibited subsequently with 2'-deoxythymidine-3', 5'-diphosphate. Nuclease-treated extracts primed with exogenous reovirus mRNA, synthesized full length polypeptides with linear kinetics for almost two hours leading to stimulation of the order of 10(4) times over endogenous background. On the average, between 40 and 50 molecules of polypeptide were synthesized per molecule of mRNA.

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