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. 1997 Sep;24(9):1774-9.

Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes

O Nemoto  1 , H Yamada, T Kikuchi, M Shinmei, K Obata, H Sato, M Seiki
Affiliations
  • PMID: 9292803

Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes

O Nemoto et al. J Rheumatol. 1997 Sep.

Erratum in

  • J Rheumatol 1998 Jan;25(1):193. Shimmei M [corrected to Shinmei M]

Abstract

Objective: To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) production by human articular chondrocytes.

Methods: Monolayer cell culture of chondrocytes was obtained from human articular cartilage from patella within 24 h after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were measured by Northern blot analysis.

Results: IL-4 suppressed IL-1 stimulated MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA.

Conclusion: IL-4 may be implicated as a protective mediator of joint destruction seen in inflammatory arthritis.

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