doi: 10.1007/s002530051043.
One-step transformation of the dimorphic yeast Yarrowia lipolytica
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- PMID: 9299782
- DOI: 10.1007/s002530051043
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One-step transformation of the dimorphic yeast Yarrowia lipolytica
Appl Microbiol Biotechnol.
1997 Aug.
Abstract
An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36-48 h after plating. The efficiency was better than 10(5) transformants/micrograms replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.
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