Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Sep;4(5):624-6.
doi: 10.1128/cdli.4.5.624-626.1997.

Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus

A S Mohamed  1 , P Becquart, H Hocini, P Métais, M Kazatchkine, L Bélec
Affiliations

Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus

A S Mohamed et al. Clin Diagn Lab Immunol. 1997 Sep.

Abstract

A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal washing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR, the genital shedding of acellular HIV RNA at the level of the mucosa itself.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. JAMA. 1993 Jun 9;269(22):2860-4 - PubMed
    1. N Engl J Med. 1994 Aug 11;331(6):341-6 - PubMed
    1. AIDS. 1996 Oct;10(12):1305-15 - PubMed
    1. J Virol Methods. 1995 Feb;51(2-3):305-16 - PubMed
    1. Am J Obstet Gynecol. 1996 Jul;175(1):122-9 - PubMed

Publication types