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. 1997 Sep;4(5):624-6.
doi: 10.1128/cdli.4.5.624-626.1997.

Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus

A S Mohamed  1 , P Becquart, H Hocini, P Métais, M Kazatchkine, L Bélec
Affiliations

Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus

A S Mohamed et al. Clin Diagn Lab Immunol. 1997 Sep.

Abstract

A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal washing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR, the genital shedding of acellular HIV RNA at the level of the mucosa itself.

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References

    1. JAMA. 1993 Jun 9;269(22):2860-4 - PubMed
    1. N Engl J Med. 1994 Aug 11;331(6):341-6 - PubMed
    1. AIDS. 1996 Oct;10(12):1305-15 - PubMed
    1. J Virol Methods. 1995 Feb;51(2-3):305-16 - PubMed
    1. Am J Obstet Gynecol. 1996 Jul;175(1):122-9 - PubMed

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