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. 1977 Oct 27;260(1):7-15.
doi: 10.1007/BF00558009.

Characterization of the synthesized collagen by mouse L-929 (L-5) cells

Characterization of the synthesized collagen by mouse L-929 (L-5) cells

S Matsubayashi et al. Arch Dermatol Res (1975). .

Abstract

In 48 h incubation L-929 (L-5) cells secreted collagenous protein accounting for 16% of the total protein. CM-chromatography, SDS-acrylamide gel electrophoresis and DEAE-cellulose chromatography revealed that the collagenous protein was mainly composed of type I collagen, having no collagen of type III. In addition, precursor-form collagen and tropocollagen molecules were found to be present in the incubate.

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