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Comparative Study
. 1997 Oct;35(10):2689-91.
doi: 10.1128/jcm.35.10.2689-2691.1997.

PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes

N A Saunders  1 , G Hallas, E T Gaworzewska, L Metherell, A Efstratiou, J V Hookey, R C George
Affiliations
Comparative Study

PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes

N A Saunders et al. J Clin Microbiol. 1997 Oct.

Abstract

A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types that were not included in the panel of capture probes were emm genotyped by sequencing.

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