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. 1976 May;17(3):281-4.

Isolation of disaturated phosphatidylcholine with osmium tetroxide

R J Mason, J Nellenbogen, J A Clements
  • PMID: 932560
Free article

Isolation of disaturated phosphatidylcholine with osmium tetroxide

R J Mason et al. J Lipid Res. 1976 May.
Free article

Abstract

A simple, rapid, inexpensive method for isolating disaturated phosphatidylcholine from adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are saturated and 94% of this material migrates as phosphatidylcholine on subsequent thin-layer chromatography.

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