Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Nov;76(11):5728-30.
doi: 10.1073/pnas.76.11.5728.

Estimation of membrane potentials of individual lymphocytes by flow cytometry

H M Shapiro, P J Natale, L A Kamentsky

Estimation of membrane potentials of individual lymphocytes by flow cytometry

H M Shapiro et al. Proc Natl Acad Sci U S A. 1979 Nov.

Abstract

The membrane potentials of individual cells can be estimated by flow cytometric quantitation of the cells' uptake of the fluorescent lipophilic cationic dye 3,3'-dihexyloxacarbocyanine iodide. Human lymphocytes separated from peripheral blood on Hypaque-Ficoll gradients are uniformly depolarized by gramicidin and hyperpolarized by valinomycin. Concanavalin A and phytohemagglutinin depolarize only a fraction of the lymphocytes. The flow cytometric technique allows precise detection of heterogeneous membrane potential responses to stimuli such as lectins; it could also provide a basis for sorting cells that respond differently to a given stimulus.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Physiol. 1976 Dec;263(2):287-319 - PubMed
    1. Biochemistry. 1975 Dec 16;14(25):5451-61 - PubMed
    1. Am J Physiol. 1976 Jul;231(1):52-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6295-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3818-22 - PubMed

LinkOut - more resources