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. 1976 May;23(2):238-41.
doi: 10.1111/j.1550-7408.1976.tb03761.x.

Axenic cultivation of Phytomonas davidi Lafont (Trypanosomatidae), a symbiote of laticiferous plants (Euphorbiaceae)

R B Mcghee, F J Postell

Axenic cultivation of Phytomonas davidi Lafont (Trypanosomatidae), a symbiote of laticiferous plants (Euphorbiaceae)

R B Mcghee et al. J Protozool. 1976 May.

Abstract

Phytomonas davidi (Trypanosomatidae) originally discovered by Lafont in 1909 on the island of Mauritius was rediscovered in Euphorbia cyathophora in Florida. Successful cultures were established in diphasic medium consisting of duck blood agar and modified Phillips' medium as overlay. Optimal growth was obtained when Mansour's medium was used as overlay and poorest growth when Cowperthwaite's medium buffered at pH 5.0 was utilized for this purpose. Marked changes tending toward choanomastigotes rather than the elongate twisted promastigotes were observed in cultures.

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