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. 1997 Sep;61(9):1580-1.
doi: 10.1271/bbb.61.1580.

Transient expression of goat growth hormone gene in poplar (Populus alba L.) protoplasts: a quick method for detection of foreign gene expression in mRNA level

J Qiao  1 , Y Ishihara, H Kuroda, F Sakai, H Sakai, T Komano
Affiliations
Free article

Transient expression of goat growth hormone gene in poplar (Populus alba L.) protoplasts: a quick method for detection of foreign gene expression in mRNA level

J Qiao et al. Biosci Biotechnol Biochem. 1997 Sep.
Free article

Abstract

We developed a sensitive, accurate, and fast method to detect foreign gene expression using reverse transcriptase-mediated polymerase chain reaction (RT-PCR) in poplar tree (Populus alba L.) protoplasts. Template total RNA was purified by removing the transfected foreign gene vector completely with DNase I treatment before the RT reaction. Expression of cDNA that encodes goat growth hormone was confirmed at the mRNA level 24 h after electroporation mediated DNA transfer.

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