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. 1997 Nov;35(11):2977-80.
doi: 10.1128/jcm.35.11.2977-2980.1997.

Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day

R K Gautom  1
Affiliations

Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day

R K Gautom. J Clin Microbiol. 1997 Nov.

Abstract

Genomic DNA patterns generated by pulsed-field gel electrophoresis are highly specific for different strains of an organism and have significant value in epidemiologic investigations of infectious-disease outbreaks. Unfortunately, time-consuming and tedious specimen processing is an inherent problem which limits the use of this powerful technology as a real-time epidemic investigational tool. Here, I describe a rapid method to improve the response time and provide specific bacterial strain identification for the typing of Escherichia coli O157:H7 and other gram-negative organisms in a single day.

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References

    1. J Clin Microbiol. 1995 Oct;33(10):2723-7 - PubMed
    1. J Clin Microbiol. 1995 Aug;33(8):2216-9 - PubMed
    1. Pediatr Infect Dis J. 1996 Nov;15(11):992-8 - PubMed
    1. J Clin Microbiol. 1990 Sep;28(9):2059-63 - PubMed
    1. Res Microbiol. 1991 Jul-Aug;142(6):667-75 - PubMed

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