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. 1997 Oct;43(3):551-6.
doi: 10.1080/15216549700204351.

Immunohistochemical analysis of heme oxygenase-I in rat liver after ischemia

K Ito  1 , S Ikeda, T Shibata, T Yano, S Horikawa
Affiliations

Immunohistochemical analysis of heme oxygenase-I in rat liver after ischemia

K Ito et al. Biochem Mol Biol Int. 1997 Oct.

Abstract

Total hepatic ischemia was induced by clamping the hepatic artery, portal vein, and bile duct. After 15 min hepatic ischemia, we examined a time course of the changes in the steady-state levels of heme oxygenase-1 (HO-1) mRNA and protein in the livers. The levels of HO-1 mRNA was transiently induced and peaked at 4 h after the start of reperfusion. In addition, a pattern of the time course of HO-1 protein levels was similar to that of HO-1 mRNA levels. Immunohistochemical analysis clearly showed that the localization of HO-1 protein induced by hepatic ischemia was restricted to the hepatocytes in the pericentral vein.

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