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. 1997 Nov;179(21):6865-70.
doi: 10.1128/jb.179.21.6865-6870.1997.

cis-Acting sequences required for light-responsive expression of the psbDII gene in Synechococcus sp. strain PCC 7942

S Anandan  1 , S S Golden
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cis-Acting sequences required for light-responsive expression of the psbDII gene in Synechococcus sp. strain PCC 7942

S Anandan et al. J Bacteriol. 1997 Nov.

Abstract

We analyzed the sequences required for promoter activity and high-light responsiveness of the psbDII gene in the cyanobacterium Synechococcus sp. strain PCC 7942 by using transcriptional fusions to a lacZ reporter gene. The basal promoter drives high constitutive expression, although no canonical -35 element is evident. The smallest fragment that showed clear light-responsive expression extends from -38 to +160, which includes 52 bp of the psbDII open reading frame. Sequences downstream from the promoter, within the untranslated leader region from +11 to +24, were required for high-light induction.

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