Plasma gonadotropin II, sex steroids, and thyroid hormones in wild striped bass (Morone saxatilis) during spermiation and final oocyte maturation

Abstract

The blood levels of gonadotropin II (GtH II), sex-steroid hormones, and thyroid hormones were determined in wild spermiating male striped bass (Morone saxatilis) in males and in females at various stages of final oocyte maturation (FOM), captured on their spawning grounds. The progression of spermiation was associated with increases in plasma GtH II and decreases in plasma testosterone (T), 11-ketotestosterone, and thyroxine (T4). Plasma triiodothyronine (T3) remained at high and relatively unchanged levels. Plasma levels of 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) and 17,20beta, 21-trihydroxy-4-pregnen-3-one (17,20beta,21-P), the proposed maturation-inducing steroids (MIS) in striped bass, were low and unchanged during the same period. It was concluded that low progestogen levels are adequate to induce spermiation in striped bass, and that higher levels may be associated with spawning behavior. In the females, based on the profiles of the studied hormones, FOM was separated into two phases. Early FOM, which included germinal vesicle (GV) migration and lipid-droplet coalescence, was associated with elevations in plasma GtH II, T, and estradiol 17beta. Late FOM, which included GV breakdown and yolk-globule coalescence, was associated with a further surge in plasma GtH II, increases in the levels of the two MIS, mainly 17, 20beta-P, and a drop in T4. Plasma T3 levels did not change during FOM. Examination of conjugated steroids demonstrated, in the males, a reduction in conjugated androgens at the peak of the spawning season and, in the females, a small increase in conjugated 17, 20beta-dihydroxylated and 5beta-reduced,3alpha-hydroxylated steroids after spawning. This is the most comprehensive report, to date, on the endocrine regulation of gonadal maturation in wild striped bass, demonstrating that a two-stage process of FOM is regulated by different endocrine signals, providing further evidence for the involvement of 17,20beta-P as a MIS in the females, and indicating that both males and females are in an euthyroid state during the spawning season.