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. 1997 Nov 1;45(3):535-40.
doi: 10.1006/geno.1997.4966.

Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)

T Yamagata  1 , T Tsuru, M Y Momoi, K Suwa, Y Nozaki, T Mukasa, H Ohashi, Y Fukushima, T Momoi
Affiliations

Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)

T Yamagata et al. Genomics. .

Abstract

The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase; EC 2. 4.1.119) (DDOST) catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. We isolated mouse and human DDOST cDNAs from retinoic acid-treated mouse P19 EC cells and human NT-2 cells, respectively. DDOST mRNA is expressed intensely in heart and pancreas, but at lower levels in brain. Here we show that the human DDOST 48-kDa subunit gene (HGMW-approved symbol DDOST) is organized into 11 exons expanding about 9 kb. This DDOST subunit gene is localized on chromosome 1p36.1 by fluorescence in situ hybridization analysis.

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