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Review
. 1997 Oct 15:825:11-22.
doi: 10.1111/j.1749-6632.1997.tb48410.x.

Modulation of apoptosis by adenosine in the central nervous system: a possible role for the A3 receptor. Pathophysiological significance and therapeutic implications for neurodegenerative disorders

M P Abbracchio  1 , S Ceruti, R Brambilla, C Franceschi, W Malorni, K A Jacobson, D K von Lubitz, F Cattabeni
Affiliations
Review

Modulation of apoptosis by adenosine in the central nervous system: a possible role for the A3 receptor. Pathophysiological significance and therapeutic implications for neurodegenerative disorders

M P Abbracchio et al. Ann N Y Acad Sci. .
No abstract available

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Figures

FIGURE 1
FIGURE 1
Induction of apoptosis by 2-CA in rat primary astrocytic cultures: light microscopy evidence. Cultures were prepared as described in Ref. and grown on glass coverslips in the absence (control not shown) or presence of the adenosine analogue (10−4 M). After 72 hr, cells were fixed, incubated with the Hoechst 33258 chromatin dye for 20 min to label nuclei, washed, immunostained with an anti-GFAP antibody as previously described to identify astroglial cells, and analyzed under a fluorescence microscope. Immunofluorescence micrographs show a GFAP staining of cultures (A) and corresponding Hoechst staining (B). Exposure to the adenosine analogue results in condensation of chromatin and appearance of apoptotic bodies (indicated by arrows in B), which are not normally detected in corresponding control cultures.
See this image and copyright information in PMC

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