doi: 10.1093/glycob/7.6.731.
The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids
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- PMID: 9376675
- DOI: 10.1093/glycob/7.6.731
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The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids
Glycobiology.
1997 Sep.
Abstract
The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. cruzi was made.
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