Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Oct 1;25(19):3957-8.
doi: 10.1093/nar/25.19.3957.

Betaine improves the PCR amplification of GC-rich DNA sequences

W Henke  1 , K Herdel, K Jung, D Schnorr, S A Loening
Affiliations

Betaine improves the PCR amplification of GC-rich DNA sequences

W Henke et al. Nucleic Acids Res. .

Abstract

Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1990 Dec 25;18(24):7465 - PubMed
    1. Biochemistry. 1993 Jan 12;32(1):137-44 - PubMed
    1. Biotechniques. 1993 Sep;15(3):372-4 - PubMed
    1. Nucleic Acids Res. 1993 Nov 11;21(22):5283-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2216-20 - PubMed