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. 1976 Apr-Jun;20(2):342-54.

Evaluation of avian mycoplasma membranes as antigens

A C Villegas, S H Kleven, D P Anderson
  • PMID: 938381

Evaluation of avian mycoplasma membranes as antigens

A C Villegas et al. Avian Dis. 1976 Apr-Jun.

Abstract

Mycoplasma gallisepticum and M. synoviae membranes were obtained by lysing mycoplasma cells with carbonate bicarbonate buffer at pH 10. Membrane preparations were compared with whole-cell preparations as to efficacy in the plate and tube agglutination and hemagglutination-inhibition tests. Membrane antigens were somewhat more sensitive in the plate and tube agglutination tests, whereas the hemagglutination-inhibition test showed no major difference between whole and membrane antigens. The membrane preparations were equal to whole-cell antigens in specificity.

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