doi: 10.1042/bj1540277.
A stopped-flow dual-wavelength spectrophotometer suitable for the study of respiratory chains
- PMID: 938452
- PMCID: PMC1172708
- DOI: 10.1042/bj1540277
Item in Clipboard
A stopped-flow dual-wavelength spectrophotometer suitable for the study of respiratory chains
Biochem J.
.
Abstract
The requirements for a dual-wavelength stopped-flow spectrophotometer to be suitable for studying limited quantities of respiratory-chain preparations are described. They can be met by a design using mainly commercially available components. The constructed apparatus has a dead-time of approx. 2.6 ms, a mixing ratio of 17:1, and a minimal requirement for 0.5 ml of mixed reactants per flow.
Similar articles
-
Kinetic characterization of the membrane-bound cytochromes of Escherichia coli grown under a variety of conditions by using a stopped-flow dual-wavelength spectrophotometer.Biochem J. 1976 Feb 15;154(2):285-94. doi: 10.1042/bj1540285. Biochem J. 1976. PMID: 180972 Free PMC article.
-
The cytochrome f-b electron-transport complex. A common link between photosynthesis and respiration in the cyanobacterium Anacystis nidulans.Biochem J. 1983 Jan 15;210(1):269-72. doi: 10.1042/bj2100269. Biochem J. 1983. PMID: 6405742 Free PMC article.
-
Effect of high pressure oxygen on the steady state of cytochromes in rat-liver mitochondria.Biochem J. 1966 Jul;100(1):254-62. doi: 10.1042/bj1000254. Biochem J. 1966. PMID: 5965254 Free PMC article.
-
[B-cytochromes in the mitochondrial respiratory chain--the role in energy conservation reaction].Tanpakushitsu Kakusan Koso. 1972 Dec;17(12):915-26. Tanpakushitsu Kakusan Koso. 1972. PMID: 4569226 Review. Japanese. No abstract available.
-
Intracellular metabolism of oxygen.Am Rev Respir Dis. 1974 Dec;110(6 Pt 2):58-63. doi: 10.1164/arrd.1974.110.6P2.58. Am Rev Respir Dis. 1974. PMID: 4374104 Review. No abstract available.
Cited by
-
Three functionally different cytochrome b redox centres in pigeon heart mitochondria.Biochem J. 1982 Feb 1;201(2):311-20. doi: 10.1042/bj2010311. Biochem J. 1982. PMID: 6123315 Free PMC article.
-
Kinetic characterization of the membrane-bound cytochromes of Escherichia coli grown under a variety of conditions by using a stopped-flow dual-wavelength spectrophotometer.Biochem J. 1976 Feb 15;154(2):285-94. doi: 10.1042/bj1540285. Biochem J. 1976. PMID: 180972 Free PMC article.
-
Sequence of b cytochromes relative to ubiquinone in the electron transport chain of Escherichia coli.J Bacteriol. 1978 Feb;133(2):477-84. doi: 10.1128/jb.133.2.477-484.1978. J Bacteriol. 1978. PMID: 203570 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
