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. 1976 Feb 15;154(2):277-84.
doi: 10.1042/bj1540277.

A stopped-flow dual-wavelength spectrophotometer suitable for the study of respiratory chains

P B Garland, S J Littleford, B A Haddock

A stopped-flow dual-wavelength spectrophotometer suitable for the study of respiratory chains

P B Garland et al. Biochem J. .

Abstract

The requirements for a dual-wavelength stopped-flow spectrophotometer to be suitable for studying limited quantities of respiratory-chain preparations are described. They can be met by a design using mainly commercially available components. The constructed apparatus has a dead-time of approx. 2.6 ms, a mixing ratio of 17:1, and a minimal requirement for 0.5 ml of mixed reactants per flow.

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References

    1. Biochem J. 1976 Feb 15;154(2):285-94 - PubMed
    1. Rev Sci Instrum. 1968 Apr;39(4):486-93 - PubMed