Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Jun 18;435(2):152-8.
doi: 10.1016/0005-2787(76)90246-x.

The mechanism of inhibition of initiation of protein synthesis in reticulocyte lysates by pyrocatechol violet

The mechanism of inhibition of initiation of protein synthesis in reticulocyte lysates by pyrocatechol violet

L J Margulies et al. Biochim Biophys Acta. .

Abstract

1. Pyrocatechol violet inhibits the initiation of protein synthesis in reticulocyte lysates. It prevents the formation of 80-S initiation complexes by inhibiting the binding of mRNA to 40-S initiation complexes. 2. Pyrocatechol violet inhibits the hydrolysis of methionyl-tRNAmetf by reticulocyte lysates or reticulocyte cell sap. 3. Concentrations of pyrocatechol violet sufficient to inhibit initiation have little effect on amino acid activation. Higher concentrations of pyrocatechol violet were strongly inhibitory.

PubMed Disclaimer

Similar articles

LinkOut - more resources