Immunological and serological data concerning human histocompatibility antigens (HLA) and alpha 1-microglobulin co-purified from urine

Abstract

Serologically active glycoproteins (HLA) purified from urine contained only about 4% HLA antigens co-purified with alpha 1-microglobulin. The immunization of rabbits with the serologically active product gave rise to an antiserum containing two kinds of antibodies: anti-HLA-A9 and anti-alpha 1-microglobulin. The anti-HLA-A9 antibodies were detected by the lymphocytotoxicity and indirect immunofluorescence techniques against peripheral blood lymphocytes and cultured lymphoid cell lines. Anti-alpha 1-microblobulin antibodies were detected by immunoprecipitation in gel. Pure alpha 1-microglobulin gave rise to an antiserum directed only against alpha 1-microglobulin. This antiserum did not react with peripheral blood lymphocytes and cultured lymphoid cell lines.