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. 1976 Jan;79(1):233-6.
doi: 10.1093/oxfordjournals.jbchem.a131053.

Enzyme coupled immunoassay of insulin using a novel coupling reagent

T Kitagawa, T Aikawa
Free article

Enzyme coupled immunoassay of insulin using a novel coupling reagent

T Kitagawa et al. J Biochem. 1976 Jan.
Free article

Abstract

A novel coupling reagent, meta-maleimidobenzoyl N-hydroxysuccinimide ester was synthesized. Using this reagent, insulin was conjugated very easily with beta-D-galactosidase [EC 3.2.1.23] in neutral, aqueous solution. No reduction of the enzyme activity was observed during the coupling procedure. The competitive bindings of the conjugate and insulin to anti-insulin serum were tested. The results indicated that the conjugate has enough immune reactivity for use in enzyme coupled immunoassay. Using this assay 20-800 pg of insulin were detectable.

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