Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Nov-Dec;8(6):785-8.
doi: 10.1021/bc970051u.

Synthesis and enzymatic stability of phosphodiester-linked peptide-oligonucleotide hybrids

J Robles  1 , M Maseda, M Beltrán, M Concernau, E Pedroso, A Grandas
Affiliations

Synthesis and enzymatic stability of phosphodiester-linked peptide-oligonucleotide hybrids

J Robles et al. Bioconjug Chem. 1997 Nov-Dec.

Abstract

Nucleopeptides Ac-Tyr(p3' dACGT)-Ala-Phe-Gly-NH2, Ac-Thr(p3'dACGT)-Ala-Phe-Gly-OH, Ac-Ser(p3'dACGT)-Ala-Phe-Gly-OH, and Phac-Hse(p3'dACGT)-Ala-Phe-Gly-OH, in which the 3'-end of a tetradeoxyribonucleotide is linked by a phosphodiester bond to a hydroxylated amino acid, were synthesized using a stepwise solid-phase methodology to study the influence of the linking amino acid on their stability to 3'-exonucleases. HPLC analysis of the reaction crudes after treatment of each nucleopeptide with snake venom phosphodiesterase showed that the lability of the amino acid-nucleoside linkage increases in the order Thr < Ser < Hse < Tyr.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources