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. 1997 Dec 19;278(5346):2123-6.
doi: 10.1126/science.278.5346.2123.

Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex

R Beckmann  1 , D Bubeck, R Grassucci, P Penczek, A Verschoor, G Blobel, J Frank
Affiliations

Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex

R Beckmann et al. Science. .

Abstract

An oligomer of the Sec61 trimeric complex is thought to form the protein-conducting channel for protein transport across the endoplasmic reticulum. A purified yeast Sec61 complex bound to monomeric yeast ribosomes as an oligomer in a saturable fashion. Cryo-electron microscopy of the ribosome-Sec61 complex and a three-dimensional reconstruction showed that the Sec61 oligomer is attached to the large ribosomal subunit by a single connection. Moreover, a funnel-shaped pore in the Sec61 oligomer aligned with the exit of a tunnel traversing the large ribosomal subunit, strongly suggesting that both structures function together in the translocation of proteins across the endoplasmic reticulum membrane.

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  • A ribosome at the end of the tunnel.
    Powers T, Walter P. Powers T, et al. Science. 1997 Dec 19;278(5346):2072-3. doi: 10.1126/science.278.5346.2072. Science. 1997. PMID: 9432719 No abstract available.
  • Tinkering with nature.
    Rehling P. Rehling P. Nat Rev Mol Cell Biol. 2011 Jun 8;12(7):401. doi: 10.1038/nrm3137. Nat Rev Mol Cell Biol. 2011. PMID: 21654705 No abstract available.

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