GABA-immunoreactive neurones and interactions of GABA with serotonin and FMRFamide in a peripheral sensory ganglion of the pond snail Lymnaea stagnalis

Abstract

The osphradium is a putative chemosensory organ of aquatic molluscs. Previously, we identified cells with serotonin (5-HT) and FMRFamide (FMRFa)-like immunoreactivity in the osphradial ganglion of Lymnaea stagnalis. The present investigation has established the presence of cells immunoreactive to gamma-aminobutyric acid (GABA). Some of these cells send processes to the sensory epithelium and are thus considered to be primary sensory neurones. Colocalisation of GABA and FMRFamide-like immunoreactivities was found in some of these and other neurones. The responses of the osphradial output electrical activity to the single and combined application of the above neuroactive substances were examined. 5-HT slightly increased and FMRFa decreased the activity. GABA alone was generally ineffective; however, it had a consistent stimulating effect after pretreatment with 5-HT. In its turn, pretreatment with GABA significantly increased the inhibitory action of FMRFa. Primary sensory neurones giving this kind of responses in the nerve were identified electrophysiologically and morphologically in the osphradial ganglion. Our results indicate that GABA takes part in relay of sensory signals into the central nervous system, and transmitter interactions involving GABA, 5-HT, and FMRFa are considerable for the final output pattern of the osphradial sensory network.