doi: 10.1126/science.279.5347.95.
Discrete start sites for DNA synthesis in the yeast ARS1 origin
Affiliations
- PMID: 9417033
- DOI: 10.1126/science.279.5347.95
Item in Clipboard
Discrete start sites for DNA synthesis in the yeast ARS1 origin
Science.
.
Abstract
Sites of DNA synthesis initiation have been detected at the nucleotide level in a yeast origin of bidirectional replication with the use of replication initiation point mapping. The ARS1 origin of Saccharomyces cerevisiae showed a transition from discontinuous to continuous DNA synthesis in an 18-base pair region (nucleotides 828 to 845) from within element B1 toward B2, adjacent to the binding site for the origin recognition complex, the putative initiator protein.
Similar articles
-
Functional equivalency and diversity of cis-acting elements among yeast replication origins.Mol Cell Biol. 1997 Sep;17(9):5473-84. doi: 10.1128/MCB.17.9.5473. Mol Cell Biol. 1997. PMID: 9271423 Free PMC article.
-
The origin recognition complex interacts with a bipartite DNA binding site within yeast replicators.Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2224-8. doi: 10.1073/pnas.92.6.2224. Proc Natl Acad Sci U S A. 1995. PMID: 7892251 Free PMC article.
-
Chromosomal ARS1 has a single leading strand start site.Mol Cell. 1999 Apr;3(4):477-86. doi: 10.1016/s1097-2765(00)80475-x. Mol Cell. 1999. PMID: 10230400
-
Replication initiation point mapping.Methods. 1997 Nov;13(3):271-80. doi: 10.1006/meth.1997.0526. Methods. 1997. PMID: 9441853 Review.
-
A protein complex present at origins of DNA replication in yeast cells.Bioessays. 1992 Aug;14(8):561-3. doi: 10.1002/bies.950140813. Bioessays. 1992. PMID: 1365910 Review. No abstract available.
Cited by
-
Molecular mechanisms of eukaryotic origin initiation, replication fork progression, and chromatin maintenance.Biochem J. 2020 Sep 30;477(18):3499-3525. doi: 10.1042/BCJ20200065. Biochem J. 2020. PMID: 32970141 Free PMC article.
-
Stable replication of papillomavirus genomes in Saccharomyces cerevisiae.J Virol. 2002 Apr;76(7):3350-8. doi: 10.1128/jvi.76.7.3350-3358.2002. J Virol. 2002. PMID: 11884560 Free PMC article.
-
The spatial arrangement of ORC binding modules determines the functionality of replication origins in budding yeast.Nucleic Acids Res. 2006;34(18):5069-80. doi: 10.1093/nar/gkl661. Epub 2006 Sep 19. Nucleic Acids Res. 2006. PMID: 16984967 Free PMC article.
-
Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene initiation zone.Mol Cell Biol. 1998 Jun;18(6):3266-77. doi: 10.1128/MCB.18.6.3266. Mol Cell Biol. 1998. PMID: 9584167 Free PMC article.
-
One-way PCR-based mapping of a replication initiation point (RIP).Nat Protoc. 2008;3(11):1729-35. doi: 10.1038/nprot.2008.173. Nat Protoc. 2008. PMID: 18927558 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
