Metabolic perturbation of the inflammatory cells

Abstract

An activation of the oxidative metabolism of phagocytes is a regular concomitant of phagocytosis as well as of other functions of these cells such as exocytosis, chemotactic locomotion, and immune-adherence. Several other substances, some of which may be found in the inflammatory site, are also able to stimulate the oxidative metabolism of phagocytes, for example phospholipases, antileucocyte antibodies, endotoxins, fatty acids, surfactants and fragments of complement. This paper deals with, (1) the molecular events occurring within the plasma membrane that trigger the stimulation of the oxidative metabolism. An experimental model is presented that involves redistribution of ions across the plasma membrane of leucocytes as the trigger of the stimulated respiration and exocytosis; (2) the intracellular localization of the enzymatic system that is the effector of the metabolic response. A scheme is proposed for the mechanism of this enzymatic system which accounts for the production of highly reactive compounds such as hydrogen peroxide, superoxide anion radical and hydroxyl radical during the metabolic activation of phagocytes.