The effect of leukocyte and platelet transfusion on the activation of intravascular coagulation by endotoxin in granulocytopenic and thrombocytopenic rabbits

Abstract

The effect of transfusion of peritoneal leukocytes, platelets, or cell suspension medium on the activation of intravascular coagulation and on the generation of capillary microclots was studied in 51 granulocytopenic and thrombocytopenic rabbits. Granulocytopenia and thrombocytopenia induced by feeding the cytoxic drug busulfan prevented the activation of intravascular coagulation and the occurrence of renal glomerular microclots after two injections of endotoxin. The transfusion of platelets into busulfan-pretreated rabbits increased the mean platelet count from 2,400 to 205,000 cells/mul, but platelet-transfused rabbits did not exhibit activation of intravascular coagulation after endotoxin injection. If however, granulocytopenic and thrombocytopenic rabbits were transfused with peritoneal leukocytes (1.0 X 10(9) cells/kg) before the second injection of endotoxin, activation of intravascular coagulation occurred, and microclot formation in renal glomerular capillaries was observed in a high percentage of animals. Positive reactions to endotoxin were obtained in leukocyte-transfused rabbits even with platelet counts of 1,000 cells/mul before the second injection of endotoxin. Thus platelets do not seem to be essentially involved in the activation of intravascular coagulation by endotoxin, whereas the presence of leukocytes is required for triggering endotoxin-induced generalized intravascular coagulation.