Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Jan;72(1):873-5.
doi: 10.1128/JVI.72.1.873-875.1998.

Glycoproteins gB, gD, and gHgL of herpes simplex virus type 1 are necessary and sufficient to mediate membrane fusion in a Cos cell transfection system

A Turner  1 , B Bruun, T Minson, H Browne
Affiliations

Glycoproteins gB, gD, and gHgL of herpes simplex virus type 1 are necessary and sufficient to mediate membrane fusion in a Cos cell transfection system

A Turner et al. J Virol. 1998 Jan.

Abstract

Herpes simplex virus type 1 glycoproteins gB, gD, and gHgL were expressed by transient transfection of Cos cells. Polykaryocyte formation above the background level seen in untransfected controls was observed only if all three components were expressed. Thus, gB, gD, and gHgL are necessary and sufficient to induce membrane fusion.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Polykaryocyte induction by HSV-1. Cos cells were transfected with the gB, gD, gH, and gL genes; the gD gene; or no DNA. Three independent counters scored the number and size of polykaryocytes with more than 10 nuclei that were observed on an 8-cm2 area, for each condition. Mean results are shown.
FIG. 2
FIG. 2
Variation in fusion scored in three experiments. Shown is the total number of nuclei in polykaryocytes with more than 10 nuclei, scored by each observer, on 8-cm2 areas transfected with plasmids expressing HSV-1 gB, gD, and gHgL or only gD.
FIG. 3
FIG. 3
Effect of omitting individual glycoproteins on polykaryocyte formation. The total number of nuclei in polykaryocytes with more than 10 nuclei was scored on 4-cm2 areas transfected with HSV-1 genes encoding gB, gD, gH, and gL, various subsets of these genes, or no DNA. The mean scores of three independent observers are plotted.
FIG. 4
FIG. 4
Ability of gH mutants to mediate fusion. (Top panel) Diagram of mutant gH proteins containing insertions at amino acid residues 691, 791, and 799. (Bottom panel) The total number of nuclei in polykaryocytes with more than 10 nuclei was scored on 4-cm2 areas transfected with plasmids expressing gB, gD, and gL, together with either wild-type or mutant gH proteins. The mean scores of two independent observers are plotted.
See this image and copyright information in PMC

References

    1. Balan P, Davis-Poynter N, Bell S, Atkinson H, Browne H, Minson T. An analysis of the in vitro and in vivo phenotypes of mutants of herpes simplex virus type 1 lacking glycoprotein gG, gE, gI or the putative gJ. J Gen Virol. 1994;75:1245–1258. - PubMed
    1. Butcher M, Raviprakash K, Ghosh H P. Acid pH-induced fusion of cells by herpes simplex virus glycoproteins. J Biol Chem. 1990;265:5862–5868. - PubMed
    1. Cai W, Gu B, Person S. Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion. J Virol. 1988;62:2596–2604. - PMC - PubMed
    1. Campadelli-Fiume G, Avitabile E, Fini S, Stirpe D, Arsenakis M, Roizman B. Herpes simplex virus glycoprotein D is sufficient to induce spontaneous pH-independent fusion in a cell line that constitutively expresses the glycoprotein. Virology. 1988;166:598–602. - PubMed
    1. Davis-Poynter N, Bell S, Minson T, Browne H. Analysis of the contributions of herpes simplex virus type 1 membrane proteins to the induction of cell-cell fusion. J Virol. 1994;68:7586–7590. - PMC - PubMed

Publication types

Substances

LinkOut - more resources