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Multicenter Study
. 1998 Jan;66(1):356-60.
doi: 10.1128/IAI.66.1.356-360.1998.

Molecular epidemiology of Staphylococcus aureus and Enterococcus faecalis in endophthalmitis

M C Booth  1 , K L Hatter, D Miller, J Davis, R Kowalski, D W Parke, J Chodosh, B D Jett, M C Callegan, R Penland, M S Gilmore
Affiliations
Multicenter Study

Molecular epidemiology of Staphylococcus aureus and Enterococcus faecalis in endophthalmitis

M C Booth et al. Infect Immun. 1998 Jan.

Abstract

Genomic DNA fingerprint analysis was performed on 39 Staphylococcus aureus and 28 Enterococcus faecalis endophthalmitis isolates collected from multiple clinical centers. Among 21 S. aureus genomic DNA fingerprint patterns identified, five clonotypes were recovered from multiple unrelated patients and accounted for 58.9% (23 of 39) of the isolates analyzed. Compared with strains having unique genomic DNA fingerprint patterns, the S. aureus clonotypes occurring more than once were more likely to result in visual acuities of 20/200 or worse (P = 0.036 [chi2 test]). In contrast to the S. aureus isolates, the E. faecalis endophthalmitis isolates were a clonally diverse population, enriched for the expression of a known toxin, cytolysin, which is plasmid encoded.

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Figures

FIG. 1
FIG. 1
PFGE of SmaI-digested chromosomal DNA of endophthalmitis-derived S. aureus clinical isolates collected from three clinical centers, BP, DM, and UP. Separate panels show clonally related isolates. Where present, subtypes are designated with the suffix 1 or 2. Molecular size standards are the New England Biolabs lambda ladder.
FIG. 2
FIG. 2
PFGE of SmaI-digested chromosomal DNA of selected endophthalmitis-derived E. faecalis clinical isolates collected from five clinical centers, BP, DM, UP, KF, and CE. Clonally related strains are identified below the panels. Molecular size standards are the New England Biolabs lambda ladder.
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References

    1. Affeldt J C, Flynn H W, Forster R K, Mandelbaum S, Clarkson J G, Jarus G D. Microbial endophthalmitis resulting from ocular trauma. Ophthalmology. 1987;94:407–413. - PubMed
    1. Bannerman T L, Rhoden D L, McAllister S K, Miller J M, Wilson L A. The source of coagulase negative staphylococci in the endophthalmitis vitrectomy study. Arch Ophthalmol. 1997;115:357–361. - PubMed
    1. Booth M C, Atkuri R V, Nanda S K, Iandolo J J, Gilmore M S. Accessory gene regulator controls Staphylococcus aureus virulence in endophthalmitis. Investig Ophthalmol Vis Sci. 1995;36:1828–1836. - PubMed
    1. Booth M C, Cheung A L, Hatter K L, Jett B D, Callegan M C, Gilmore M S. Staphylococcal accessory regulator (sar) contributes to Staphylococcus aureus virulence in endophthalmitis only in conjunction with agr. Infect Immun. 1997;65:1550–1556. - PMC - PubMed
    1. Brinton G S, Topping T M, Hyndiuk R A, Aaberg T M, Reeser F H, Abrams G W. Posttraumatic endophthalmitis. Arch Ophthalmol. 1984;102:547–550. - PubMed

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