doi: 10.1111/j.1432-1033.1976.tb10111.x.
Formation of uridine diphosphate 2-deoxy-D-glucose and guanosine diphosphate 2-deoxy-D-glucose in vitro using animal enzymes
- PMID: 942918
- DOI: 10.1111/j.1432-1033.1976.tb10111.x
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Formation of uridine diphosphate 2-deoxy-D-glucose and guanosine diphosphate 2-deoxy-D-glucose in vitro using animal enzymes
Eur J Biochem.
.
Free article
Abstract
The enzymic formation of guanosine diphosphate 2-deoxy-D-glucose and uridine diphosphate 2-deoxy-D-glucose from synthetically prepared 2-deoxy-D-glucose 1-phosphate is described. Incubation of 2-deoxy-D-glucose 1-phosphate with an enzyme preparation from bovine mammary glands and either GTP or UTP gives rise to the corresponding nucleoside-diphosphate derivative of 2-deoxy-D-glucose. Uridine diphosphate 2-deoxy-D-glucose could also be obtained by incubation of 2-deoxy-D-glucose 1-phosphate with UTP and UDP glucose pyrophosphorylase from beef liver.
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