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. 1998 Jan;36(1):251-4.
doi: 10.1128/JCM.36.1.251-254.1998.

Development of an immunomagnetic method for selective isolation of Actinobacillus pleuropneumoniae serotype 1 from tonsils

A Gagné  1 , S Lacouture, A Broes, S D'Allaire, M Gottschalk
Affiliations

Development of an immunomagnetic method for selective isolation of Actinobacillus pleuropneumoniae serotype 1 from tonsils

A Gagné et al. J Clin Microbiol. 1998 Jan.

Abstract

An immunomagnetic separation technique (IMS) for the selective isolation of Actinobacillus pleuropneumoniae serotype 1 was developed. Superparamagnetic polystyrene beads (immunomagnetic beads [IMBs]) were coated with purified rabbit immunoglobulin G specific for A. pleuropneumoniae serotype 1. The antibody concentration, the number of IMBs, the incubation time, and the temperature of incubation influenced the recovery of the target bacteria. The sensitivity of the IMS technique was 1,000-fold higher than that of direct culture. When tonsils from animals from infected herds were tested, significantly more positive tonsils were detected by the IMS technique (68%) than by the standard procedures (22%). The method represents an innovative and highly sensitive approach for the isolation of A. pleuropneumoniae from carrier animals.

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References

    1. Biswas B, Vemulapalli R, Dutta S K. Detection of Ehrlichia risticii from feces of infected horses by immunomagnetic separation and PCR. J Clin Microbiol. 1994;32:2147–2151. - PMC - PubMed
    1. Bossé J T, Johnson R P, Rosendal S. Serodiagnosis of pleuropneumonia using enzyme-linked immunosorbent assay with capsular polysaccharide of serotypes 1, 2, 5 and 7. Can J Vet Res. 1990;54:427–431. - PMC - PubMed
    1. Chapman P A, Wright D J, Siddons C A. A comparison of immunomagnetic separation and direct culture for the isolation of verotoxin-producing Escherichia coli O157 from bovine faeces. J Med Microbiol. 1994;40:424–427. - PubMed
    1. Fenwick B W, Osburn B I. Immune responses to the lipopolysaccharides and capsular polysaccharides of Haemophilus pleuropneumoniae in convalescent and immunized pigs. Infect Immun. 1986;54:575–582. - PMC - PubMed
    1. Fratamico P M, Schultz F J, Buchanan R L. Rapid isolation of Escherichia coli O157:H7 from enrichment cultures of foods using an immunomagnetic separation method. Food Microbiol. 1992;9:105–113.

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