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. 1998 Jan;64(1):356-8.
doi: 10.1128/AEM.64.1.356-358.1998.

Biodegradation of phosphonomycin by Rhizobium huakuii PMY1

J W McGrath  1 , F Hammerschmidt, J P Quinn
Affiliations

Biodegradation of phosphonomycin by Rhizobium huakuii PMY1

J W McGrath et al. Appl Environ Microbiol. 1998 Jan.

Abstract

The biodegradation by Rhizobium huakuii PMY1 of up to 10 mM phosphonomycin as a carbon, energy, and phosphorus source with accompanying P(i) release is described. This biodegradation represents a further mechanism of resistance to this antibiotic and a novel, phosphate-deregulated route for organophosphonate metabolism by Rhizobium spp.

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Figures

FIG. 1
FIG. 1
Structure of phosphonomycin (cis-1,2-epoxypropylphosphonic acid; fosfomycin).
FIG. 2
FIG. 2
Growth of isolate PMY1 on phosphonomycin (5 mM) as the sole carbon, energy, and phosphorus source. Cultures were incubated at 30°C on an orbital shaker at 100 rpm. Growth was measured by the increase in optical density at 650 nm, while phosphate release was determined by the method of Fiske and SubbaRow (4). Symbols: •, growth (optical density at 650 nm); ▪, percentage of phosphate released from substrate.
FIG. 3
FIG. 3
Stationary-phase cell yields of isolate PMY1 as a function of phosphonomycin concentration (0 to 20 mM).
See this image and copyright information in PMC

References

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