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. 1998 Jan;180(2):426-9.
doi: 10.1128/JB.180.2.426-429.1998.

Construction and characterization of a Helicobacter pylori clpB mutant and role of the gene in the stress response

E Allan  1 , P Mullany, S Tabaqchali
Affiliations

Construction and characterization of a Helicobacter pylori clpB mutant and role of the gene in the stress response

E Allan et al. J Bacteriol. 1998 Jan.

Abstract

Antiserum raised against whole Helicobacter pylori cells identified a novel 94-kDa antigen. The nucleotide sequence of the gene encoding the 94-kDa antigen was determined, and analysis of the deduced amino acid sequence revealed structural features typical of the ClpB ATPase family of stress response proteins. An isogenic H. pylori clpB mutant showed increased sensitivity to high-temperature stress, indicating that the clpB gene product functions as a stress response protein in H. pylori.

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Figures

FIG. 1
FIG. 1
Immunoblot analysis of E. coli XLOLR containing either pBK-CMV with a 3.2-kb insert of H. pylori genomic DNA (CP6) or pBK-CMV alone (CP1). Blots were probed with rabbit anti-H. pylori whole-cell antiserum and detected with alkaline phosphatase-conjugated anti-rabbit antibody. The numbers to the left indicate the sizes of the protein standards in kilodaltons. The arrow to the right indicates the 94-kDa antigen expressed by E. coli CP6.
FIG. 2
FIG. 2
Survival at 50°C of wild-type H. pylori (N6) and the H. pylori clpB mutant (N6.1). Median values for five experiments are shown.
See this image and copyright information in PMC

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