Comparative Study
doi: 10.1006/meth.1997.0524.
Mapping replication origins, pause sites, and termini by neutral/alkaline two-dimensional gel electrophoresis
Affiliations
- PMID: 9441851
- DOI: 10.1006/meth.1997.0524
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Comparative Study
Mapping replication origins, pause sites, and termini by neutral/alkaline two-dimensional gel electrophoresis
Methods.
1997 Nov.
Abstract
Neutral/alkaline two-dimensional gel electrophoresis is a robust, easily interpretable, sensitive technique that has yielded insights about the in vivo replication of many types of DNA, from multicopy yeast plasmids to single-copy chromosome regions in unsynchronized mammalian cells. It can provide information about directions of replication fork movement and locations of replication origins, termini, and pause sites. Especially when combined with its partner technique, neutral/neutral two-dimensional gel electrophoresis, it is a method of choice for investigation of unknown situations.
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