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Comparative Study
. 1997 Nov;14(10):624-8.
doi: 10.1023/a:1022545121367.

A new method for the purification of human motile spermatozoa applying density-gradient centrifugation: polysucrose media compared to Percoll media

C Y Andersen  1 , J Grinsted
Affiliations
Comparative Study

A new method for the purification of human motile spermatozoa applying density-gradient centrifugation: polysucrose media compared to Percoll media

C Y Andersen et al. J Assist Reprod Genet. 1997 Nov.

Abstract

Purpose: A newly developed method for the isolation of human motile spermatozoa using density-gradient centrifugation was compared with the traditionally used Percoll technique.

Method: Sperm samples were divided into two equal aliquots, which were purified with either the traditionally performed Percoll technique or a new alternative based on polysucrose/Optiprep media. For each sample the isolation was performed during the same run of the centrifuge.

Results: The average recovery of progressively motile spermatozoa with the polysucrose/Optiprep method was significantly higher (48 +/- 7%) than with the Percoll method (38 +/- 6%) (n = 18). The average percentage of motile spermatozoa and the motility score were similar in the purified preparations.

Conclusions: The new polysucrose/Optiprep-based density-gradient centrifugation technique for the isolation of motile human spermatozoa is as good as the traditionally used Percoll method and may replace it in connection with assisted reproduction techniques.

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References

    1. Mortimer D. Sperm preparation techniques and iatrogenic failures of in-vitro fertilization. Hum Reprod. 1991;6:173–176. - PubMed
    1. Lessley BA, Garner DL. Isolation of motile spermatozoa by density gradient centrifugation in Percoll. Gamete Res. 1983;7:49.
    1. Jaroudi KA, Carver-Ward JA, Hamilton CJCM, Sieck UV, Sheth KV. Percoll semen preparation in male-factor infertility as shown by a randomized cross-over study. Hum Reprod. 1993;8:1438–1342. - PubMed
    1. Aitken RJ. Pathophysiology of human spermatozoa. Curr Opin Obstet Gynecol. 1994;6:128–135. - PubMed
    1. Aitken RJ, Clarkson JS. Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. J Androl. 1988;9:367–376. - PubMed

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