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. 1998 Feb;66(2):874-7.
doi: 10.1128/IAI.66.2.874-877.1998.

Siderophore production by cystic fibrosis isolates of Burkholderia cepacia

P Darling  1 , M Chan, A D Cox, P A Sokol
Affiliations

Siderophore production by cystic fibrosis isolates of Burkholderia cepacia

P Darling et al. Infect Immun. 1998 Feb.

Abstract

Sixty-one Burkholderia cepacia isolates from patients with cystic fibrosis (CF) and four plant isolates were screened for production of the siderophores salicylic acid (SA), pyochelin, cepabactin, and ornibactins and fingerprinted by a PCR-based randomly amplified polymorphic DNA (RAPD) method. Of the 24 RAPD types determined, 22 (92%) were associated with isolates that produced SA, 21 (87%) were associated with isolates that produced ornibactins, 15 (60%) were associated with isolates that produced pyochelin, and 3 (12%) were associated with isolates that produced cepabactin. Of the 24 RAPD types plus 2 phenotypic variants of types 1 and 9, 3 were associated with isolates that produced all four siderophores, 8 were associated with isolates that produced three siderophores, 12 were associated with isolates that produced two siderophores, and 3 were associated with isolates that produced only one siderophore. These results suggest that the numbers and types of siderophores produced by CF isolates of B. cepacia correlate with RAPD type and that SA and ornibactins are the most prevalent siderophores produced.

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Figures

FIG. 1
FIG. 1
RAPD fingerprints of DNAs from B. cepacia isolates amplified with primer 208. Lane 1, molecular size markers (1-kb ladder); lane 2, control reaction without DNA; lane 3, type 1; lane 4, type 2; lane 5, type 3; lane 6, type 3A; lane 7, type 4; lane 8, type 4A; lane 9, type 5; lane 10, type 6; lane 11, type 7; lane 12, type 8; lane 13, type 9; lane 14, molecular size markers (100-bp ladder; sizes are shown on the right). A photograph of the gel was scanned with a Hewlett-Packard Scan Jet 4C scanner with Hewlett-Packard Deskscan II software.
See this image and copyright information in PMC

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