Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1998 Jan;5(1):33-7.
doi: 10.1128/CDLI.5.1.33-37.1998.

Age-stratified seroprevalence of neutralizing antibodies to astrovirus types 1 to 7 in humans in The Netherlands

M P Koopmans  1 , M H Bijen, S S Monroe, J Vinjé
Affiliations
Comparative Study

Age-stratified seroprevalence of neutralizing antibodies to astrovirus types 1 to 7 in humans in The Netherlands

M P Koopmans et al. Clin Diagn Lab Immunol. 1998 Jan.

Abstract

Astroviruses are a new family of positive-stranded RNA viruses that cause gastroenteritis in a wide range of animals and in humans. Seven types of astrovirus, tentatively considered serotypes, have been distinguished by enzyme-linked immunosorbent assays (ELISA) or immunoelectron microscopy, but it is unclear whether the serotype designation is used properly. To test human sera for the presence of neutralizing antibodies and to type field strains, neutralization tests (NT) using CaCo2 tissue-culture-adapted astrovirus strains 1 to 7 and the corresponding rabbit reference sera were developed. In rabbits, neutralizing antibodies were predominantly serotype specific, with the exception of low-level cross-reactivity in astrovirus serotype 4 reference serum with astrovirus serotype 1 virus. Similarly, in humans, no evidence of cross-reactivity was found for the serotype combinations tested (all except the combination 1 and 7 and the combination 6 and 7). Typing by NT was concordant with typing by ELISA and genotyping, with one exception. The seroprevalence rates of neutralizing antibodies in an age-stratified sample of the population in Utrecht Province (n = 242) were 91% for astrovirus serotype 1, 69% for astrovirus serotype 3, 56% for astrovirus serotype 4, 36% for astrovirus serotype 5, 31% for astrovirus serotype 2, 16% for astrovirus serotype 6, and 10% for astrovirus serotype 7. Acquisition of antibodies was slower among persons seropositive for astrovirus serotype 5 than among those seropositive for astrovirus serotypes 1 to 4, suggesting that the epidemiology of serotype 5 astrovirus is different from that of astrovirus serotypes 1 to 4.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Age-stratified seroprevalence (percentage of sera tested per age group) of neutralizing antibodies to astrovirus serotypes 1 to 7.
FIG. 2
FIG. 2
Fraction of positive sera per titer level for astrovirus serotypes 1 to 5.
FIG. 3
FIG. 3
Age-stratified distribution (fraction of astrovirus-positive sera per group) of high (>160) and low (=20) titers of antibody to astrovirus serotype 1.
See this image and copyright information in PMC

References

    1. Echeverria P, Hoge C W, Bodhidatta L, Tungtaem C, Herrmann J, Imlarp S, Tamura K. Etiology of diarrhea in a rural community in western Thailand: importance of enteric viruses and enterovirulent Escherichia coli. J Infect Dis. 1994;169:916–919. - PubMed
    1. Grandien M, Forsgren M, Ehrnst A. Enteroviruses and reoviruses. In: Schmidt N J, Emmons R W, editors. Diagnostic procedures for viral, rickettsial, and chlamydial infections. 6th ed. Washington, D.C: APHA Inc.; 1989. pp. 513–578.
    1. Herrmann J E, Hudson R W, Perron-Henry D M, Kurtz J B, Blacklow N R. Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies. J Infect Dis. 1988;158:182–185. - PubMed
    1. Hudson R W, Herrmann J E, Blacklow N R. Plaque quantitation and virus neutralization assays for human astroviruses. Arch Virol. 1989;108:33–38. - PubMed
    1. Jiang B, Monroe S S, Koonin E V, Stine S E, Glass R I. RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis. Proc Natl Acad Sci USA. 1993;90:10539–10543. - PMC - PubMed

Publication types

MeSH terms

Substances