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. 1998 Jan;5(1):70-3.
doi: 10.1128/CDLI.5.1.70-73.1998.

Heterogeneity of immunoglobulin G response to Helicobacter pylori measured by the unweighted pair group method with averages

K Mayo  1 , S Pretolani, G Gasbarrini, G Ghironzi, F Megraud
Affiliations

Heterogeneity of immunoglobulin G response to Helicobacter pylori measured by the unweighted pair group method with averages

K Mayo et al. Clin Diagn Lab Immunol. 1998 Jan.

Abstract

The heterogeneity of the immune response to Helicobacter pylori has always been noticed but has never been evaluated by obtaining a quantitative measure. For this purpose, sera were tested by enzyme-linked immunosorbent assay, and 207 positive serum specimens were subsequently tested by immunoblotting. The presence or absence of six specific bands was noted. The homology of the different profiles of bands was measured by calculating the Dice coefficient, and a dendrogram was constructed. Thirty-four profiles were found, with each profile containing from 1 to 43 serum specimens. At a level of 72% similarity, 115 of the serum specimens studied fell into eight profiles. At a level of 48% similarity, 186 of the serum specimens studied fell into 22 profiles. The difference in immunoblot profiles was probably linked to the host immune response, but infection with strains carrying different antigens cannot be ruled out.

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Figures

FIG. 1
FIG. 1
Dendrogram of 34 different profiles (combinations of six bands) revealed by immunoblotting of 207 serum specimens which had tested positive for H. pylori antibodies by ELISA.
FIG. 2
FIG. 2
Number of serum specimens in each profile.
See this image and copyright information in PMC

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