Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Jan;5(1):70-3.
doi: 10.1128/CDLI.5.1.70-73.1998.

Heterogeneity of immunoglobulin G response to Helicobacter pylori measured by the unweighted pair group method with averages

K Mayo  1 , S Pretolani, G Gasbarrini, G Ghironzi, F Megraud
Affiliations

Heterogeneity of immunoglobulin G response to Helicobacter pylori measured by the unweighted pair group method with averages

K Mayo et al. Clin Diagn Lab Immunol. 1998 Jan.

Abstract

The heterogeneity of the immune response to Helicobacter pylori has always been noticed but has never been evaluated by obtaining a quantitative measure. For this purpose, sera were tested by enzyme-linked immunosorbent assay, and 207 positive serum specimens were subsequently tested by immunoblotting. The presence or absence of six specific bands was noted. The homology of the different profiles of bands was measured by calculating the Dice coefficient, and a dendrogram was constructed. Thirty-four profiles were found, with each profile containing from 1 to 43 serum specimens. At a level of 72% similarity, 115 of the serum specimens studied fell into eight profiles. At a level of 48% similarity, 186 of the serum specimens studied fell into 22 profiles. The difference in immunoblot profiles was probably linked to the host immune response, but infection with strains carrying different antigens cannot be ruled out.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Dendrogram of 34 different profiles (combinations of six bands) revealed by immunoblotting of 207 serum specimens which had tested positive for H. pylori antibodies by ELISA.
FIG. 2
FIG. 2
Number of serum specimens in each profile.
See this image and copyright information in PMC

References

    1. Andersen L P. The antibody response to Helicobacter pylori infection, and the value of serologic tests to detect H. pylori and for post-treatment monitoring. In: Goodwin C S, Worsley B W, editors. Helicobacter pylori: biology and clinical practice. Boca Raton, Fla: CRC Press, Inc.; 1993. pp. 285–305.
    1. Andersen L P, Esperson F. Immoglobulin G antibodies to Helicobacter pylori in patients with dyspeptic symptoms investigated by the Western immunoblot technique. J Clin Microbiol. 1992;30:1743–1751. - PMC - PubMed
    1. Bayerdörfer E, Oertel H, Lehn N, Kasper G, Nannes G A, Sauerbruch T, Stolte M. Topographic association between active gastritis and Campylobacter pylori colonization. J Clin Pathol. 1989;42:834–839. - PMC - PubMed
    1. Burnette W N. Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein. Anal Biochem. 1981;56:480–488. - PubMed
    1. Censini S, Lange C, Xiang Z, Crabtree J E, Ghiara P, Borodovsky M, Rappioli R, Covacci A. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc Natl Acad Sci USA. 1996;93:14548–14653. - PMC - PubMed