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. 1998 Feb;64(2):795-9.
doi: 10.1128/AEM.64.2.795-799.1998.

Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA

J R Marchesi  1 , T Sato, A J Weightman, T A Martin, J C Fry, S J Hiom, D Dymock, W G Wade
Affiliations

Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA

J R Marchesi et al. Appl Environ Microbiol. 1998 Feb.

Erratum in

  • Appl Environ Microbiol 1998 Jun;64(6):2333

Abstract

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

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References

    1. Amann R I, Ludwig W, Schleifer K-H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev. 1995;59:143–169. - PMC - PubMed
    1. Ausubel F M, Brent R, Kingston R E, Moore D D, Seidman J G, Smith J A, Struhl K. Current protocols in molecular biology. New York, N.Y: Greene Publishing Associates-Wiley Interscience; 1989.
    1. Baranton, G. Personal communication.
    1. Brosius J, Palmer J L, Kennedy H P, Noller H F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA. 1978;75:4801–4805. - PMC - PubMed
    1. Brunk C F, Avaniss-Aghajani E, Brunk C A. A computer analysis of primer and probe hybridization potential with bacterial small-subunit rRNA sequences. Appl Environ Microbiol. 1996;62:872–879. - PMC - PubMed

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