Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Feb;64(2):800-3.
doi: 10.1128/AEM.64.2.800-803.1998.

Purification and characterization of anti-Listeria compounds produced by Geotrichum candidum

V Dieuleveux  1 , D Van Der Pyl, J Chataud, M Gueguen
Affiliations

Purification and characterization of anti-Listeria compounds produced by Geotrichum candidum

V Dieuleveux et al. Appl Environ Microbiol. 1998 Feb.

Abstract

Geotrichum candidum can produce and excrete compounds that inhibit Listeria monocytogenes. These were purified by ultrafiltration, centrifugal partition chromatography, thin-layer chromatography, gel filtration, and high-pressure liquid chromatography, and analyzed by liquid chromatography-mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and optical rotation. Two inhibitors were identified: D-3-phenyllactic acid and D-3-indollactic acid.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Reverse-phase liquid chromatography (HPLC) of inhibitory compounds of G. candidum after purification by centrifugal partition chromatography, preparative TLC, and Sephadex LH20 gel filtration. Column, C18 Grom-Sil ODS2 column (4.6 by 30 mm; particle size, 1.5 μm; Grom Analytic). Eluent: solvent A (0.1% formic acid in water), solvent B (CH3CN-H2O [95:5] plus 0.1% formic acid). Flow rate, 1 ml/min. (a) Product 1 (detection at 206 nm); (b) product 2 (detection at 222 nm).
FIG. 2
FIG. 2
Structure of two inhibitory compounds of G. candidum characterized by LC-mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and optical rotation.
See this image and copyright information in PMC

References

    1. Betina V. Bioautography in paper and thin layer chromatography and its scope in the antibiotic field. J Chromatogr. 1973;78:41–51. - PubMed
    1. Borde V. La Listeria bouleverse l’hygiène alimentaire. Ind Tech. 1993;735:19–20.
    1. Dieuleveux, V., J. Chataud, and M. Guéguen. Inhibition of Listeria monocytogenes by Geotrichum candidum. Submitted for publication.
    1. Doyle M P. Effect of environmental and processing conditions on Listeria monocytogenes. Food Technol. 1988;42:169–171.
    1. George S M, Lund B M, Brocklehurst T F. The effect of pH and temperature on initiation of growth of Listeria monocytogenes. Lett Appl Microbiol. 1988;6:153–156.

Publication types

MeSH terms

LinkOut - more resources