Melatonin controls superoxide anion level: modulation of superoxide dismutase activity in ring dove heterophils
- PMID: 9468113
- DOI: 10.1111/j.1600-079x.1998.tb00360.x
Melatonin controls superoxide anion level: modulation of superoxide dismutase activity in ring dove heterophils
Abstract
The pineal gland and the immune system in mammals and birds are functionally connected, with some recent findings showing that melatonin, the major secretory product of the pineal gland in vertebrates, is a free-radical scavenger and general antioxidant. In birds, heterophils, with their ability to ingest and kill different antigens, play a central role in the host defense mechanism. This in vitro study investigated the possible antioxidant action of melatonin in heterophils isolated from adult male and female ring dove (Streptopelia risoria). The superoxide anion levels (O2-.) and the superoxide dismutase (SOD) activity were evaluated as indicators of the metabolic burst of heterophils after ingestion of inert particles (latex beads) at the basal time (0 min) and 15, 30, 45, and 60 min of incubation with 100 microM melatonin. The heterophil O2-. levels, measured by the nitroblue tetrazolium reduction test, were lower after incubation in the presence of melatonin at all the times studied, a time-dependent effect. Latex beads produced a significant increase in SOD activity, whereas there was a significant decrease in the presence of melatonin. In addition, when heterophils were incubated in the presence of both latex beads and melatonin, the indole appeared to annul the enhancement of SOD activity (U-525nm) produced by latex beads. In conclusion, our findings show that the neurohormone melatonin could be a useful antioxidant.
Similar articles
-
Phagocytosis of Candida albicans and superoxide anion Levels in ring dove (Streptopelia risoria) heterophils: effect of melatonin.J Neuroendocrinol. 2003 Dec;15(12):1111-5. doi: 10.1111/j.1365-2826.2003.01103.x. J Neuroendocrinol. 2003. PMID: 14636172
-
Suppression of both basal and antigen-induced lipid peroxidation in ring dove heterophils by melatonin.Biochem Pharmacol. 1999 Oct 15;58(8):1301-6. doi: 10.1016/s0006-2952(99)00207-5. Biochem Pharmacol. 1999. PMID: 10487532
-
Melatonin and the phagocytic process of heterophils from the ring dove (Streptopelia risoria).Mol Cell Biochem. 1997 Mar;168(1-2):185-90. doi: 10.1023/a:1006850518225. Mol Cell Biochem. 1997. PMID: 9062908
-
Suppression of oxygen toxicity by melatonin.Zhongguo Yao Li Xue Bao. 1998 Nov;19(6):575-81. Zhongguo Yao Li Xue Bao. 1998. PMID: 10437151 Review.
-
Pharmacological actions of melatonin in oxygen radical pathophysiology.Life Sci. 1997;60(25):2255-71. doi: 10.1016/s0024-3205(97)00030-1. Life Sci. 1997. PMID: 9194681 Review.
Cited by
-
Melatonin and tryptophan counteract lipid peroxidation and modulate superoxide dismutase activity in ringdove heterophils in vivo. Effect of antigen-induced activation and age.Age (Dordr). 2009 Sep;31(3):179-88. doi: 10.1007/s11357-009-9107-2. Epub 2009 Jul 10. Age (Dordr). 2009. PMID: 19590980 Free PMC article.
-
A review of the multiple actions of melatonin on the immune system.Endocrine. 2005 Jul;27(2):189-200. doi: 10.1385/ENDO:27:2:189. Endocrine. 2005. PMID: 16217132 Review.
-
Antioxidant neuroprotection against ethanol-induced apoptosis in HN2-5 cells.Brain Res. 2009 Aug 18;1285:14-21. doi: 10.1016/j.brainres.2009.06.029. Epub 2009 Jun 16. Brain Res. 2009. PMID: 19538946 Free PMC article.
-
Comparative study of the heterophil phagocytic function in young and old ring doves (Streptopelia risoria) and its relationship with melatonin levels.J Comp Physiol B. 2004 Jul;174(5):421-7. doi: 10.1007/s00360-004-0429-1. Epub 2004 May 18. J Comp Physiol B. 2004. PMID: 15148621
-
Assessment of the Potential Role of Tryptophan as the Precursor of Serotonin and Melatonin for the Aged Sleep-wake Cycle and Immune Function: Streptopelia Risoria as a Model.Int J Tryptophan Res. 2009;2:23-36. doi: 10.4137/ijtr.s1129. Epub 2009 Jan 14. Int J Tryptophan Res. 2009. PMID: 22084580 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical